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cellscript公司全系列產品
Cellscript™的使命是為臨床研究和治療提供*的產品和技術，用于制造和使用RNA在細胞中進行翻譯。
目前的產品包括用于體外轉錄的試劑盒、使用cap模擬物或cap酶的5' RNA capping和3' RNA聚腺苷酸化的試劑盒，以及用于制造用于細胞翻譯的cap、poly(a)-tail mRNA的all-in-one試劑盒。
Cellscript™近推出了隱身™的體外合成RNA的RNA轉錄套件包含修飾核苷,如假尿苷(Ψ)和/或5-methylcytidine (m5C)代替相應的U或C規(guī)范核苷(圖1)。這些工具都取名為“因為封頂,聚腺苷酸和核苷修飾的RNA產物(稱為“INCOGNITO mRNAs”)是經過偽裝的，因此當它們被轉染到表達多種RNA傳感器的哺乳動物細胞中時，不會像相應的未經修飾的mrna一樣，引發(fā)先天免疫反應。
產品清單：
cellscript公司全系列產品
mRNA Production
T7 mScript™ Standard mRNA Production System (PDF)
| Simply the best way to make in vitro mRNA. Combines the benefits of high-yield transcription with those of post-transcriptional capping and tailing all in one kit. See T7-FlashScribe Transcription Kit, ScriptCap m7G Capping System, ScriptCap 2'-O-Methyltransferase Kit and A-Plus Poly(A) Polymerase Tailing Kit on this page. |
| C-MSC11610 | 10 reactions | $320 |
| C-MSC100625 | 25 reactions | $702 |
In Vitro Transcription
T7-FlashScribe™ Transcription Kit (PDF)
| For the production of in vitro transcribed RNA with T7 RNA polymerase and canonical (GAUC) NTPs. A reaction produces 180 µg RNA from 1 µg DNA in 30 minutes. |
| C-ASF3507 | 50 reactions | $286 |
T7-Scribe™ Standard RNA IVT Kit (PDF)
| For the production of in vitro transcribed RNA with T7 RNA polymerase and canonical (GAUC) NTPs. A reaction produces 150 µg RNA from 1 µg DNA in 2 hours. |
| C-AS3107 | 50 reactions | $267 |
SP6-Scribe™ Standard RNA IVT Kit (PDF)
| For the production of in vitro transcribed RNA with SP6 RNA polymerase and canonical (GAUC) NTPs. A reaction produces 90 µg RNA from 1 µg DNA in 2 hours. |
| C-AS3106 | 50 reactions | $267 |
Post-Transcriptional Capping
ScriptCap™ m7G Capping System (PDF)
| For post-transcriptional capping of in vitro transcribed RNA with ScriptCap Capping Enzyme, GTP and SAM. Cap 0 (N7-methyl-G) caps can be built with efficiencies approaching *. This cannot be accomplished with co-transcriptional capping methods. Use in conjunction with ScriptCap 2'-O-Methyltransferase to produce Cap 1 caps, that which is found on most eukaryotic mRNAs. |
| C-SCCE0625 | 25 reactions | $436 |
ScriptCap™ 2'-O-Methyltransferase Kit (PDF)
| For conversion of RNA containing Cap 0 caps to those containing Cap 1 caps, which are found on most eukaryotic mRNAs. Can be used simultaneously with ScriptCap m7G Capping System to produce virtually * Cap 1-capped RNA in a single reaction. |
| C-SCMT0625 | 25 reactions | $219 |
ScriptCap™ Cap 1 Capping System (PDF)
| For post-transcriptional capping of in vitro transcribed RNA with ScriptCap Capping Enzyme, ScriptCap 2'-O-Methyltransferase, GTP and SAM. Either Cap 0 or Cap 1 caps can be built with efficiencies approaching *. This cannot be accomplished with co-transcriptional capping methods. |
| C-SCCS1710 | 10 reactions | $211 |
Co-Transcriptional Capping
MessageMAX™ T7 ARCA-Capped Message Transcription Kit (PDF)
| For co-transcriptional capping of IVT RNA with T7 RNA polymerase and Anti-Reverse cap analog (ARCA). A reaction produces 60 µg RNA from 1 µg DNA in 30 minutes. Reaction products are 80% correctly capped and 20% uncapped. |
| C-MMA60710 | 10 reactions | $323 |
AmpliCap-Max™ T7 High Yield Message Maker Kit (PDF)
| For co-transcriptional capping of IVT RNA with T7 RNA polymerase and standard cap analog. A reaction produces 60 µg RNA from 1 µg DNA in 30 minutes. Reaction products are 40% correctly capped, 40% incorrectly capped and 20% uncapped. |
| C-ACM04037 | 25 reactions | $368 |
AmpliCap™ SP6 High Yield Message Maker Kit (PDF)
| For co-transcriptional capping of IVT RNA with SP6 RNA polymerase and standard cap analog. A reaction produces 35 µg RNA from 1 µg DNA in 2 hours. Reaction products are 40% correctly capped, 40% incorrectly capped and 20% uncapped. |
| C-AC0706 | 25 reactions | $368 |
Post-Transcriptional Tailing
A-Plus™ Poly(A) Polymerase Tailing Kit (PDF)
| For post-transcriptional poly(A) tail addition to RNAs. Allows tailing of mRNAs in vitro, to lengths equivalent to or greater than those found in nature. This cannot be accomplished with co-transcriptional tailing methods. |
| C-PAP5104H | 50 reactions | $219 |
Modified NTP In Vitro Transcription
INCOGNITO™ T7 Ψ-RNA Transcription Kit (PDF)
| For the production of pseudouridine-containing, in vitro transcribed RNA with T7 RNA polymerase. Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 100 µg RNA from 1 µg DNA in 2 hours. |
| C-ICTY110510 | 10 reactions | $410 |
INCOGNITO™ SP6 Ψ-RNA Transcription Kit (PDF)
| For the production of pseudouridine-containing, in vitro transcribed RNA with SP6 RNA polymerase. Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 60 µg RNA from 1 µg DNA in 2 hours. |
| C-ICSY110510 | 10 reactions | $410 |
INCOGNITO™ T7 5mC- & Ψ-RNA Transcription Kit (PDF)
| For the production of 5-methylcytosine- pseudouridine-containing, in vitrotranscribed RNA with T7 RNA polymerase. 5mC- Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 100 µg RNA from 1 µg DNA in 2 hours. |
| C-ICTMY110510 | 10 reactions | $691 |
INCOGNITO™ T7 ARCA 5mC- & Ψ-RNA Transcription Kit (PDF)
| For co-transcriptional capping of 5-methylcytosine- pseudouridine-containing, in vitro transcribed RNA with T7 RNA polymerase and Anti-Reverse cap analog (ARCA). 5mC- Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 40 µg RNA from 1 µg DNA in 3 hours. Reaction products are 80% correctly capped and 20% uncapped. |
| C-ICTAMY110510 | 10 reactions | $842 |
mRNA Decapping
Cap-Clip™ Acid Pyrophosphatase (PDF)
| For the removal of cap structures (decapping) from various RNAs (i.e. mRNAs) resulting in 5'-monophosphate RNAs. Can be substituted unit for unit, into any preexisting protocol for Tobacco Acid Pyrophosphatase (TAP) utilizing the same buffer. |
| C-CC15011H | 100 units | $550 |
RNase Inhibition
ScriptGuard™ RNase Inhibitor (PDF)
| Inhibits eukaryotic RNases A, B and C. Use as a safeguard against RNA degradation in any RNA-containing reaction. |
| C-SRI6310K | 10,000 units | $278 |
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