thermo二抗A11032|Invitrogen抗體*

thermo二抗A11032|Invitrogen抗體*

Alexa Fluor染料是目前值得信賴的熒光染料之一。 Invitrogen™Alexa Fluor 594染料是一種明亮的紅色熒光染料，具有理想的激發(fā)，適用于594 nm激光線。為了在成像和流式細(xì)胞儀中產(chǎn)生穩(wěn)定的信號，Alexa Fluor 594染料在很寬的摩爾范圍內(nèi)對pH不敏感。具有高熒光量子產(chǎn)率和高光穩(wěn)定性的探針允許以高靈敏度檢測低豐度生物結(jié)構(gòu)。 Alexa Fluor 594染料分子可以高摩爾比附著在蛋白質(zhì)上而不會發(fā)生明顯的自猝滅，從而實(shí)現(xiàn)更明亮的結(jié)合物和更靈敏的檢測。每種綴合物的標(biāo)記程度通常為每個(gè)IgG分子2-8個(gè)熒光團(tuán)分子;每個(gè)產(chǎn)品批次的分析證書上都標(biāo)明了確切的標(biāo)簽程度。

為了使交叉反應(yīng)性小化，這些山羊抗小鼠IgG（H + L）全二級抗體已被親和純化并且針對牛IgG，山羊IgG，兔IgG，大鼠IgG，人IgG和人血清交叉吸附。交叉吸附或預(yù)吸附是提高抗體特異性的純化步驟，導(dǎo)致更高的靈敏度和更少的背景染色。使第二抗體溶液通過含有來自潛在交叉反應(yīng)性物質(zhì)的固定化血清蛋白的柱基質(zhì)。在柱中僅捕獲非特異性結(jié)合的二抗，并且高度特異性的第二抗體流過。這種額外步驟的好處在多重/多色染色實(shí)驗(yàn)（例如，流式細(xì)胞術(shù)）中是顯而易見的，其中存在與其他一抗的潛在交叉反應(yīng)性或在可能存在內(nèi)源性免疫球蛋白的組織/細(xì)胞熒光染色實(shí)驗(yàn)中。

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

1-10 µg/mL

Immunocytochemistry (ICC)

2 µg/mL

Immunofluorescence (IF)

2 µg/mL

Published Applications

Immunohistochemistry (Frozen) (IHC (F))

See 5 publications below

Immunohistochemistry (IHC)

See 6 publications below

Immunocytochemistry (ICC)

See 7 publications below

Immunohistochemistry (Paraffin) (IHC (P))

See 1 publication below

Miscellaneous PubMed (MISC)

See 191 publications below

Product Specifications

Species Reactivity

Mouse

Host / Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobins Heavy and Light chains

Conjugate

Alexa Fluor® 594

Excitation/Emission Profile

View spectra 

Form

Liquid

Concentration

2 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.5

Contains

5mM sodium azide

Storage conditions

4° C, store in dark

RRID

AB_2534091

Target

IgG

Cross Adsorption

Against bovine IgG, goat IgG, rabbit IgG, rat IgG, human IgG and human serum

Antibody Form

Whole Antibody